FortebioBiosensor-YourSolutionforHigh-ThroughputBiomolecularAnalysis

Biomolecularanalysisplaysacrucialroleinunderstandingbiologicalprocesses,developingnewdrugs,anddiagnosingdiseases.However,traditionaltechniquessuchasELISA,Westernblotting,andgelelectrophoresisaretime-consuming,labor-intensive,andmaylacksensitivityandspecificity.Therefore,manyresearchershaveturnedtobiosensorsasanalternativemethodforbiomolecularanalysis.Fortebio'sbiosensortechnologyoffersareliableandhigh-throughputsolutionforcharacterizingbiomoleculesinvariousapplications.

TheScienceBehindFortebioBiosensors

Fortebiobiosensorsarebasedontheprincipleofsurfaceplasmonresonance(SPR),whichdetectschangesinrefractiveindexcausedbybiomolecularinteractions.Thebiosensorconsistsofasensorchip,whichhasathingold-coatedsurfacetocreateaSPRphenomenon,andaninstrument,whichilluminatesthesensorchipwithamonochromaticlightsourceandmeasuresthereflectedlightintensityinreal-time.Whenabiomoleculebindstothesurfaceofthesensorchip,italterstherefractiveindexandcausesashiftintheSPRsignal,providinginformationaboutthekineticsandaffinityoftheinteraction.

Thebiosensorcandetectabroadrangeofbiomolecules,includingproteins,antibodies,nucleicacids,lipids,andcarbohydrates,invarioussampletypessuchasserum,celllysates,culturesupernatants,andcrudeextracts.Furthermore,Fortebiooffersavarietyofsensorchipformatsandassayconfigurationstooptimizeassayperformancefordifferentapplicationsandtargets.

AdvantagesofFortebioBiosensors

Fortebiobiosensorsofferseveraladvantagesovertraditionalmethodsforbiomolecularanalysis:

• Speed:Biosensorassayscanbecompletedinminutestohoursinsteadofdaystoweeks,allowingforhigh-throughputandreal-timeanalysis.
• Sensitivity:Biosensorscandetectbiomolecularinteractionswithfemtomolartopicomolaraffinities,whichisseveralordersofmagnitudehigherthanELISAandWesternblotting.
• Specificity:Biosensorscandiscriminatebetweencloselyrelatedmoleculesbasedontheirbindingkineticsandspecificity,whichiscriticalfordrugdiscoveryandclinicaldiagnostics.
• Flexibility:Biosensorscanbeusedforawiderangeofapplications,includingantibodyscreening,protein-proteininteractions,smallmoleculeanalysis,andqualitycontrol.

ApplicationsofFortebioBiosensors

Fortebiobiosensorshavebeenusedinvariousfieldsofresearchandindustry,suchas:

• Drugdiscovery:Biosensorscanidentifyandoptimizedrugcandidatesbyscreeningforbindingaffinity,kinetics,andspecificity.
• Proteinengineering:Biosensorscancharacterizeprotein-proteinorprotein-ligandinteractionstoimproveproteinstability,function,andselectivity.
• Qualitycontrol:Biosensorscanmonitorthepurity,identity,andpotencyofbiopharmaceuticalstoensureproductsafetyandefficacy.
• Clinicaldiagnostics:Biosensorscandetectdiseasebiomarkersinpatientsamplesfordiagnosis,prognosis,andmonitoring.
• Agricultureandfoodsafety:Biosensorscandetectmicrobialcontaminants,pesticides,andtoxinsinfoodandenvironmentalsamplesforfoodsafetyandbiosecurity.

Insummary,Fortebiobiosensorsprovideaversatileandreliablesolutionforhigh-throughputbiomolecularanalysis.Withtheirspeed,sensitivity,specificity,andflexibility,biosensorshavebecomeanindispensibletoolforacceleratingresearchanddevelopmentinvariousfields.